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Analytical ultracentrifugation
| Protein | sAB | KD1 | sABB | KD2 |
|---|---|---|---|---|
| S | μm | S | μm | |
| Gadkin (Δ51) WT | 2.71 | 0.042 | 5.57 | 162 |
| Gadkin (Δ51) Y277A, F280A | 2.85 | 3.04 | 117 | 15215 |
| Gadkin (Δ51) W260L, F264L | 2.63 | 104 | 3.14 | 107 |
| Gadkin (Δ51) W260L, F264L, Y277A, F280A | 2.71 (fixed) | NDa | 2.05 | ND |
a ND, not determined.
