FIGURE 4.
Gfr but not Efr was required for fucose modifications of N-glycans. A–C, AAL staining (magenta) of the late third instar wing discs. A, a wild-type wing disc. B, a wing disc of Gfr1/Gfr1. C, a wing disc of Efr1/Y. All confocal images were obtained under the same conditions. D–F, a wing disc of Gfr1/Gfr1 overexpressing Efr under the control of en-Gal4. D, AAL staining (magenta). E, anti-GFP antibody staining (green) showing the region overexpressing Efr. F, a merged image of D and E. G, Western blot analysis of the total protein extracts prepared from the imaginal discs and central nervous system of wild-type (Wt), Efr1/Y (Efr1), Gfr1/Gfr1 (Gfr1), Efr1/Y;; Gfr1/Gfr1 (Efr1; Gfr1), and GmdH78/GmdH78 (GmdH78) mutant larvae. α-1,3-fucose modifications were detected by anti-HRP antibody staining. Bar, 100 μm.