FIGURE 1.

Cystitis increases the urinary bladder weight and the thickness of the bladder wall. After CYP treatment for 2, 8 or 48 h, the urinary bladder was dissected out, and the weight was measured. The paraformaldehyde-fixed bladder was sectioned at a thickness of 10 μm and stained by hematoxylin and eosin. Cystitis caused significant increases in petechial hemorrhages (A) and in the weight (B) of the urinary bladder starting from 2 h following CYP treatment. Hematoxylin and eosin staining shows edema, infiltration and considerable damage of the urothelium (C). The thickness of the bladder wall was significantly increased from 2 h post-CYP injection (D). Results were presented as mean ± S.E. from 4–5 animals at each time point. *, p < 0.05 versus control. Bar, 500 μm.