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. 2009 Dec 7;285(6):4206–4212. doi: 10.1074/jbc.M109.040444

FIGURE 2.

FIGURE 2.

Increases in the type I collagen mRNA and protein expression levels in the urinary bladder following CYP-induced cystitis. After CYP treatment, the urinary bladder was homogenized for RNA or protein extraction. The urothelium and smooth muscle layers were also separated and homogenized for RNA extraction. Real-time PCR results showed that the type I collagen mRNA level was increased at 2–8 h post-CYP treatment and returned to normal level at 48 h post-CYP treatment (A). At 8 h of cystitis, the level of type I collagen mRNA was increased in the smooth muscle but not urothelium of the inflamed bladder (B). Western blot (C) showed that the type I collagen protein level was increased at 8 h and 48 h post CYP treatment (D). Results were presented as mean ± S.E. from 3–5 animals at each time point. *, p < 0.05 versus control.