FIGURE 3.

Changes in Akt, JNK, and p38 MAPK phosphorylation levels in the urinary bladder during cystitis. Following CYP treatment, the Akt phosphorylation level was examined by Western blot (A) and showed a significant increase in the inflamed bladder at 8 h (B). The JNK phosphorylation level was also increased (C, D, and E) in the inflamed bladder as that the 55-kDa isoform of JNK1 was increased at 8–48 h post-CYP treatment (D), while the 46-kDa isoform of JNK1 was increased at 8 h post-CYP treatment (E). Western blot (F) showed that the phosphorylation level of p38 MAPK was not changed in the inflamed bladder. Results were presented as mean ± S.E. from 3–6 animals at each time point. *, p < 0.05 versus control.