FIGURE 5.

Attenuation of cystitis-induced Akt and ERK1/2 phosphorylation in the urinary bladder by NGF antibody. In the control IgG-treated animals, cystitis increased the phosphorylation levels of Akt (A and B) and ERK1/2 (C and D) in the inflamed bladder. Administration of NGF neutralizing antibody had no effects on the basal level of phospho-Akt and phospho-ERK1/2 in the urinary bladder but significantly blocked cystitis-induced up-regulation of phospho-Akt and phospho-ERK1/2 in the inflamed bladder. Results were presented as mean ± S.E. from 3 animals at each time point. *, p < 0.05 versus IgG; #, p < 0.05 versus CYP+IgG.