FIGURE 9.

Up-regulation of type I collagen expression in the urinary bladder smooth muscle by NGF. In cultured detrusor smooth muscle, the level of type I collagen mRNA was significantly increased by NGF treatment (100 ng/ml) for 1 h. Pretreatment for 30 min with PD98059 (1 μm) or wortmannin (0.5 μm) attenuated NGF-induced type I collagen expression. PD98059 or wortmannin alone had no effects on the level of type I collagen mRNA expression. The results were from 3 independent experiments and presented as mean ± S.E. *, p < 0.05 versus 0 treatment; #, p < 0.05 versus NGF treatment for 1 h.