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. 2009 Dec 8;285(6):4224–4231. doi: 10.1074/jbc.M109.081018

FIGURE 7.

FIGURE 7.

Celastrol induction of p23 fibrils in wild-type and mutant p23. A, ability of wild-type full-length p23 and its core domain p23C35 (residues 1–119) to chaperone PR was compared with that of p23 having the mutation C58S or the three mutations C40S, C58S, and C75S (Triple). 4P represents reconstitution of PR with Hsp90, Hsp70, Hop, and Hsp40 (Ydj1). Data represent three experiments in duplicate. Cel, celastrol. B, fibril formation upon celastrol treatment of p23 triple mutant (C40S, C58S, and C75S) was monitored by electron microscopy. C, ability of dihydrocelastrol to induce p23 fibrils was tested by thioflavin-T (ThT) binding using inhibitor-to-protein ratios of 2, 4, or 8 as indicated. Data represent three experiments. D, ability of dihydrocelastrol to induce p23 fibrils was monitored by electron microscopy.