FIGURE 5.

The specificity and domains of Zili-Smad4 interaction. A, schematic of different Zili deletion constructs. B–D, interactions between HA-Smad4 and different Zili deletion mutants with FLAG tag. E, schematic of different Smad4 deletion constructs. F, interactions between FLAG-Zili and different Smad4 deletion mutants with HA tag. The assays were performed in human HEK293 cells, TCL indicates total cell lysate, which is consistently used in Fig. 6. WB, Western blot; IP, immunoprecipitation; WT, wild type.