Figure 5. Lack of NFATc3 protein or in vivo treatment with A-285222 prevents OPN induction.

A. Blood glucose levels in BalB/c mice treated with STZ or vehicle. Mice received i.p. injections once a day of A-285222 or equal volumes of saline (control). B. Summarized data and representative immunoblot from western blot experiments showing OPN expression normalized to β-actin in the aorta of mice treated as in A (N=7-8 in each group). **P < 0.01 and ***P < 0.001 vs. untreated control. C. Blood glucose levels in NFATc3+/+ and NFATc3-/- mice treated with STZ or vehicle. D. Summarized data and representative immunoblot from western blot experiments showing OPN expression normalized to β-actin in the aorta of mice treated as in C (N=6-8 in each group).