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. 2010 Feb 2;123(5):699–711. doi: 10.1242/jcs.058511

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Fig. 8. — ISG75 protein and mRNA after dhh1-E182Q induction. (A) ISG75 protein expression was determined by western blotting over a time course after dhh1-E182Q induction. A titration of bloodstream-form (BSF) cell equivalents was used to estimate expression levels. BiP expression is shown as a loading control (Bangs et al., 1993). (B) Distribution of ISG75 mRNA on a polysome analysis before and 12 hours after dhh1-E182Q induction analysed by northern blotting. The distributions shown are relative with the total set at 100%. eIF4E3 mRNA was used as a control.