Fig. 3.
The Tsc1-Tsc2 complex and Rhb1 are involved in the regulation of p27 phosphorylation. Extracts were subjected to immunoblotting with the indicated antibodies. (A) JUp1204 (WT) and PJ001 (tsc2Δ) were grown and treated as described in Fig. 2A. (B) Cells of 972 (WT) and PJ001 (tsc2Δ) were treated with EMM-N (a nitrogen-free version of EMM) as described in Fig. 2B. (C) JUp1188 (WT), JUp1190 (rhb1V17A) and JUp1194 (rhb1K120R) were grown and treated as described in Fig. 2A. The amount of myc-Rhb1 was examined by anti-myc antibody. (D) JUp1188 (WT) and JUp1190 (rhb1V17A) were treated with EMM-N as described in Fig. 2B.