Fig. 4.

General protein synthesis but not thyroglobulin expression increases early during stimulation. FRTL5 cells were deprived of hormones for 5 days, after which they were stimulated by the addition of 6H. At the indicated time points, cells were pulse-labeled for 30 minutes and lysed. Post-nuclear lysate, normalized to total amount of protein, was separated on 6% and 10% SDS-PA gels. (A) Autoradiograph of the 6% gel (upper panel; ³⁵S signal), and the corresponding Tg western blot (lower panel; ECL signal). The arrow indicates Tg. Total signal and Tg signal were quantified using the Image Quant software package using non-saturated exposures of 6% and 10% SDS-PA gels. (B) Histogram showing the mean values expressed as a change relative to label incorporation before stimulation for both total protein and Tg. (C) Percentage of total label incorporated into Tg after different stimulation times.