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. 2010 Jan 25;7:3. doi: 10.1186/1742-4690-7-3

Figure 4.

Figure 4

Analysis of TVA and TVB bridge proteins. (A) Schematic representation of TVA and TVB fusion proteins. The ends of the unprocessed TVA and TVB extracellular domains including their signal peptide sequences are shown. To generate TVA-Epo and TVB-Epo, the human Epo sequence encoding amino acids 28 to 193 of the mature protein was placed downstream of the linker-encoding sequence. The numbers refer to the ends of the respective protein domains. (B) Western blot analysis of TVA and TVB fusion proteins. Cell supernatants collected from 293T cells stably expressing TVA and TVB proteins were analyzed by Western blotting using monoclonal anti-V5 antibody. Lanes 1, 2, 4, and 5: Supernatants from cells producing TVA, TVB, TVB-Epo and TVA-Epo proteins, respectively. Lane 3: Control 293T cells. The amounts loaded were equalized based on the V5 epitope-specific ELISA assay described in Materials and Methods.