Table 1.
Comparison of titers of lentiviral vectors pseudotyped with ALV-A or ALV-B Env glycoproteins in cell lines expressing TVA and TVB receptors, EpoR or c-kit
| Vector pseudotypes | Cells | Titers (TU/ml)d |
|---|---|---|
| ALV-A | 293 DK-7a | 1.57 ± 0.47 × 108 |
| ALV-A/Epo | 293 or 293T 293T-EpoRb |
<104 8.60 ± 0.70 × 107 |
| ALV-A/SCF | MO7-ec | 1.17± 0.25 × 107 |
| ALV-B | 293 DK-7a | 1.20 ± 0.30 × 107 |
| ALV-B/Epo | 293 or 293T 293T-EpoRb |
<104 9.2 ± 2.6 × 107 |
| ALV-B/SCF | MO7-ec | 8.38 ± 3.60 × 106 |
aTransduction efficiencies of ALV-A and ALV-B pseudotypes were determined on 293 DK-7 cells, a clonal cell line expressing the chimeric TVA/TVB receptor. Vectors used were concentrated 250-fold. The titers of un-concentrated virus were 8.12 ± 4.4 × 105 (ALV-A) and 2.0 ± 0.9 × 105(ALV-B) respectively.
bPseudotypes preloaded with TVA-Epo or TVB-Epo were used to transduce a 293T-based clonal cell line expressing EpoR. Vectors used were concentrated 250-fold. The titers of un-concentrated virus were 7.27 ± 0.77 × 105 (ALV-A) and 7.80 ± 0.46 × 105 (ALV-B) respectively.
cPseudotypes preloaded with TVA-SCF or TVB-SCF were used to transduce c-kit-expressing MO7-e cells. Vectors used were concentrated 250-fold.
dTiters presented represent the mean ± SD from three independent experiments using concentrated vector stocks.