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Tol2 based luciferase reporter constructs to read-out circadian molecular oscillation in mammalian cells. Bmal1:luc-pT2A construct contains 0.5 kb-long mouse Bmal1 promoter driven luciferase cDNA and hygromycin B-resistant cassette (Hyg+). The Dbp:luc-pT2A construct contains hygromycin B-resistant cassette (Hyg+) and 0.6 kb-long mouse Dbp promoter driven luciferase cDNA (see Methods).
