Figure 2.

Heat shock alters the expression of different sets of genes in wild-type and PPARα-null mice. A. Direct comparison of HS genes in wild-type and PPARα-null mice. The relatively small number of genes which exhibited altered expression in both strains are shown in detail. B. Different classes of HS genes. The genes altered by HS were divided into six classes (I - VI) based on expression in wild-type and PPARα-null mice and comparison with WY. The genes whose expression was confirmed by TaqMan are underlined. Red, up-regulation; green, down-regulation; black, no change. The intensity scales indicates fold-change due to exposure relative to controls. C. Altered expression of genes assessed by TaqMan. There were four animals per treatment group, and each sample was analyzed in duplicate. Variability is expressed as standard error of the mean. Means and S.E. (n = 4) for RT-PCR data were calculated by Student's t test. The level of significance was set at p ≤ 0.05.