Table 2.
Estimation of gene copy number of antimicrobial effectors and non immune gene by real time PCR
| gene | Sample | Primer efficiency (E) | Calculated copy number | Estimated copy number | ||
|---|---|---|---|---|---|---|
| Mean1 | SD | Mean2 | SD | |||
| Cg-bpi | pDNA | 2.01 | ± 0.006 | |||
| gDNA | 2.06 | ± 0.060 | 0.95 | ± 0.10 | 1 | |
| Cg-prp | pDNA | 1.99 | ± 0.008 | |||
| gDNA | 2.03 | ± 0.040 | 12.59 | ± 0.99 | 11 - 13 | |
| Cg-defs | pDNA | 2.02 | ± 0.004 | |||
| gDNA | 2.03 | ± 0.010 | 47.46 | ± 6.4 3 | 41 - 53 | |
| Cg-actin | pDNA | 1.99 | ± 0.030 | |||
| gDNA | 2.07 | ± 0.040 | 168.04 | ± 10. 4 | 156 - 176 | |
gDNA: genomic DNA from C. gigas
pDNA: plasmid DNA used as standard
1 Mean of primer efficiency (E = 10-slope) from three technical replicates
2 Mean of three biological replicates
SD: standard deviation