Table 3.
List of primers
| Sense primers (5'3') | Antisense primers (5'3') | |||
|---|---|---|---|---|
| gene | PCR amplification from cDNA and gDNA | |||
| Cg-prp | Cg-prp2F | CACCATGTTCTCTCGGAGGA | Cg-prp3R | TCTCTTCATCAAAACAAAGTCG |
| Cg-defm | Cg-defm5F | CCACTTTCTGGTTTGCTGAG | Cg-defU4R | TCTTGGTCAGATTCAGWCTGG |
| Cg-defh | Cg-defh4F | CTACCAGTTGTTCATACAGAG | Cg-defU4R | TCTTGGTCAGATTCAGWCTGG |
| Cg-bpi | Cg-bpi1F | CTACCAGTTGTTCATACAGAG | Cg-bpi1R | GGATTTAATATATCCGTCTTCTG |
| Cg-actin | Cg-actin1F | CTTCACAATGGGAGATGAAGA | Cg-actin1R | GTAAACTCCTATCACAGCCAC |
| gene copy number estimation | ||||
| Cg-prp | Cg-prpUq1F | CCACCATGTTCTCTCGGAGG | Cg-prpUq1R | CTTGTAGAACGGGCTAGCAC |
| Cg-defs | Cg-defUq1F | CTGCAGACATGGCTTTTGCTG | Cg-defUq1R | GTACATCTTGACCAGAGCGTG |
| Cg-bpi | Cg-bpiq2F | AGATAGAAATAGGAATGGACGG | Cg-bpiq2R | GTTATAGATCCACGCTGCTCC |
| Cg-actin | Cg-actq2F | CAGCTATGTAGGAGACGAG | Cg-actq3R | CACGGAGTTCATTGTAGAAGG |
| 5' and ' RACE | ||||
| Cg-defh | Cg-defh3'sp1 | GCTGGATTTGGGTGTCCG | race (dT) anchor | GACCACGCGTATCGATGTCGAC |
| Cg-defh | Cg-defh5' sp1 | CACAGTAGCCCGCTCTACA | ||
| Cg-defh | oligo (dT) anchor | GACTCGAGTCGACATCGATTTTTTTTTTTTTTTTV | Cg-defh5' sp2 | CGGACACCCAAATCCAGC |
| Cg-defh | race (dT) anchor | GACCACGCGTATCGATGTCGAC | Cg-defh5' sp3 | CCATYTCTGCAGAAACCATC |
| Cg-defm | Cg-defm5' sp1 | CATCTTAACCAGAGCGTGGC | ||
| Cg-defm | oligo (dT) anchor | GACTCGAGTCGACATCGATTTTTTTTTTTTTTTTV | Cg-defm5' sp2 | GGACTTGCAGTGATTGTTGC |
| Cg-defm | race (dT) anchor | GACCACGCGTATCGATGTCGAC | Cg-defm5' sp3 | CATCAGAAGGACAGCTAGTG |