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Adaptation of culture conditions of aNS-1 cells to microplates. aNS-1 cells can be cultured in 96- (A) and 384-well (B) plates, maintaining the expression of proper NS cells markers (Nestin, Vimentin, BLBP, Olig2), without exiting the cell cycle (Phospho-Histone3 immunoreactivity, P-H3), without differentiation (beta III-tubulin, MAP2 and GFAP absence) or cell death (cleaved caspase-3 absence).
