Table 3.
Relative proportion of aNS-1 cells expressing neural progenitor, neuronal and glial antigens during differentiation in 96- and 384-well microplates
| Plate format | Nestin | beta III-tubulin | MAP2 | GFAP | |
|---|---|---|---|---|---|
| DIV3 | 96W 384W |
6.06 ± 3.34 (n = 608) 5.51 ± 3.45 (n = 155) |
57.12 ± 14.03 (n = 661) 58.16 ± 7.14 (n = 904) |
63.80 ± 8.12 (n = 608) 69.49 ± 5.07 (n = 882) |
0.55 ± 30.61 (n = 661) 0.10 ± 0.30 (n = 904) |
| DIV7 | 96W 384W |
1.36 ± 1.05 (n = 659) n.a. |
59.91 ± 16.55 (n = 695) 58.35 ± 6.20 (n = 999) |
61.31 ± 7.59 (n = 659) 66.82 ± 9.47 (n = 738) |
2.13 ± 1.36 (n = 695) 1.30 ± 1.04 (n = 999) |
| DIV14 | 96W 384W |
3.36 ± 3.61 (n = 715) 2.84 ± 1.05 (n = 788) |
59.45 ± 9.59 (n = 636) 62.54 ± 22.95 (n = 763) |
87.29 ± 6.77 (n = 715) 71.53 ±19.17 (n = 788) |
4.94 ± 1.48 (n = 636) 5.82 ± 2.65 (n = 763) |
| DIV21 | 96W 384W |
5.36 ± 3.40 (n = 598) 6.00 ± 4.76 (n = 447) |
68.38 ± 11.07 (n = 573) 80.89 ± 14.57 (n = 484) |
90.80 ± 14.67 (n = 598) 98.76 ± 16.00 (n = 333) |
7.70 ± 3.29 (n = 573) 9.22 ± 2.49 (n = 484) |
Data were evaluated by Attovision software analysing aNS-1 alive cells expressing neuronal (beta III-tubulin, MAP2), neural progenitor (Nestin) and glial (GFAP) markers; 96- and 384-well microplates experiments were run in parallel; n: number of cells analyzed.