Figure 8. Intranasal adoptive transfer of iCD8⁺DC, but not iCD8⁻DC, enhanced IFN- γ production by T cell.

Mice were adoptively transferred i.n. with iCD8⁺DC or iCD8⁻DC subsets followed by the challenge with BCG through i.n route as described in Materials and Methods. Draining lymph nodes was isolated aseptically and single cell suspension was prepared in cold staining buffer. Cells were co-stained with FITC-anti CD3ε, PE-anti CD4, PerCP-anti CD8 Abs and stained for intracellular IFN-γ using allophycocyanin -conjugated anti-IFNγ Ab as described in Materials and Methods. Cells were gated on CD3ε⁺T cells, CD3ε⁺CD4⁺T cell and CD3ε⁺CD8⁺T cell respectively and the percentage of positive cells for IFN-γ is indicated in upper right corner (A). Pooled data in each group are shown as mean±SD (B). One representative experiment of two independent experiments is shown. *p<0.05; **, p<0.01.