Figure 1. ZNF9 specifically binds to a human cellular mRNA IRES element.

A. Increasing amounts of recombinant, full-length ZNF9 were incubated with ³²P-end-labeled ODC RNA probe and cross-linked. Reactions were separated by polyacrylamide gel electrophoresis, transferred to nitrocellulose and exposed to X-ray film. Membranes were stained for ZNF9 protein using Ponceau S. B. Recombinant ZNF9 was incubated with ³²P-end-labeled ODC RNA probe and either a 50-fold molar excess of bovine liver tRNA or a 50-fold molar excess of unlabeled ODC RNA competitor (comp) probe. Samples were processed as in A.