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. 2010 Feb 18;5(2):e9301. doi: 10.1371/journal.pone.0009301

Figure 4. Cap-independent translation of ODC is defective in DM2 myoblasts.

Figure 4

A. IRES activity in wild-type and DM2 myoblast. Equal numbers of human wild-type or DM2 myoblasts were infected with virions expressing the bicistronic ODC reporter vector and were analyzed for luciferase activity. The level of cap-independent translation is reported as a ratio of cap-independent translation (firefly) to cap-dependent translation (Renilla). Values are averages of 6 independent experiments and are normalized to IRES activity in wild-type myoblasts. B. Differences in ZNF9 mRNA expression in wild-type and DM2 myoblasts. Quantitative real-time PCR was performed on equal amounts of total mRNA from wild-type and DM2 myoblasts. Values were computed using the ΔΔCt method and are normalized to levels of GAPDH mRNA. Error bars represent the S.E.M. C. IRES activity in wild-type and DM2 myoblast overexpressing ZNF9. Equal numbers of wild-type or DM2 myoblasts were infected with the bicistronic ODC reporter vector and vectors for either the overexpression of LacZ or ZNF9. Cell lysates were analyzed for luciferase activity as described above. Values are reported as a ratio of cap-independent translation to cap-dependent translation and are representative of 4 independent experiments. The values are normalized to the value of IRES activation in wild-type myoblasts overexpressing LacZ. Error bars represent the S.E.M.