Skip to main content
. 2010 Feb 18;5(2):e9290. doi: 10.1371/journal.pone.0009290

Figure 1. hESC derived NPs express neural crest PNS markers and PNS related growth factor receptors.

Figure 1

(A) Schematic flow diagram of the differentiation protocol of hESC derived NPs along 10 weeks culture. (B) RT-PCR analysis of mRNA extracts from hNPs at 5 and 8 weeks in suspension cultures express various early neural crest specific markers prior to final differentiation. Expression of the housekeeping gene GAPDH served as reaction control for each sample. (C–H) Cross section of 5 weeks old NPs in suspension cultures expressing the migratory-crest marker HNK-1 throughout the sphere section (D), while at the edge of the sphere section colocalization of the PNS neuronal marker peripherin and the pan neuronal marker β-Tubulin III is observed (C, F and G respectively). Scale bars are indicated in A and C. (I) Comparative RT-PCR analysis of mRNA extracts from hNPs at 3 and 8 weeks in suspension cultures express different pattern of neuronal differentiation-related growth factor receptors. Expression of the housekeeping gene RPL-27 served as reaction control for each sample.