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. 2010 Feb 18;5(2):e9290. doi: 10.1371/journal.pone.0009290

Figure 2. In vitro PNS differentiation capacity of hESC derived NPs.

Figure 2

(A–S) Eight weeks old Human NPs cultured on laminin for 12 days showing extensive neurite outgrowth expressing the pan neuronal marker β-tubulin III (A–D). These cells also express the PNS marker Peripherin (H,H,K,N and Q) together with the neural crest marker HNK-1 (F), the synaptic vesicle marker SV2 (I) and vesicular acetylcholine transporter VAChT (L), indicative of their maturity, the PNS markers Brn3a (O) and Istet-1 (R). (T) Quantitative analysis from these experiments (N–R) of the proportion of peripherin positive cells from total cells in the culture and the relative numbers of double stained Peripherin+/Brn3a+ and Peripherin+/Islet-1+ from total Peripherin+ cells. (Scale bar: in A-B = 0.5 mm; in C-S = 50 µm).