Figure 3. Electrophysiological analysis of NPs derived PNS neurons.

The current-voltage (IV) relationship acquired from cell in voltage clamp mode, holding potential was −60 mV (A, upper panel). The inward current deflections are the sodium currents evoked by 200 ms-long step depolarization from −60 to +40 mV with a 20 mV increment (A, lower panel). Representative traces of single action potential (B, left panel) and repetitive action potential firing (B, right panel) acquired from the cell in current clamp mode by 2 ms and 200 ms-long suprathreshold current injection (B, left and right lower panels, respectively). Increase in intracellular calcium concentration evoked by high KCl application (C), Representative trace from fura-2 AM-loaded cell in response to local 70 mM KCl application is indicated by arrows. These experiments were repeated 4 times, using cells from different cultures each time, obtaining the same results.