Figure 4. Suppressed expression of nucleoporins decreases c-Fos expression in carcinoma cells.

OVCAR-10 and SKOV3 ovarian and MCF7 breast cancer cells were transiently transfected with siRNA constructs to Nup153 and Nup62, as well as a control siRNA (from Santa Cruz). After 48 h, cells were serum-starved overnight, then stimulated with 20% FBS for 90 min, and lysed for immunoblot analysis. NPC proteins were detected with a mouse pan-anti-NPC monoclonal antibody.