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. Author manuscript; available in PMC: 2010 Feb 17.
Published in final edited form as: Am J Physiol Cell Physiol. 2002 Mar;282(3):C451–C460. doi: 10.1152/ajpcell.00333.2001

Fig. 7.

Fig. 7

Intracellular localization of 130-kDa MLCK in BPAE cells. BPAE cells transiently expressing Fl-130-kDa MLCK were fixed and reacted with mouse anti-Flag antibody and Alexa 488-conjugated goat-anti-mouse secondary antibody to detect MLCK or with affinity-purified anti-NMHC-IIA or -IIB antibodies and Alexa 568-conjugated goat anti-rabbit IgG as indicated. Each vertical row represents the same cell expressing 130-kDa MLCK and merged images are shown in C and F (MLCK in green; NMHC-IIA or -IIB in red; overlap is yellow-orange). The staining pattern for the 130-kDa MLCK is predominantly cytoplasmic, although fine filaments are detectable that colocalize with NMHC-IIA but do not coincide with NMHC-IIB.