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Intracellular localization of 220-kDa MLCK in BPAE cells. BPAE cells transiently expressing GFP-220-kDa MLCK were fixed and incubated antibodies to detect NMHC-IIA or NMHC-IIB. Each vertical row represents the same cell expressing 220-kDa MLCK, and merged images are shown in C and F (MLCK in green; NMHC-IIA or -IIB in red). The filamentous staining pattern of the 220-kDa MLCK does not colocalize with either NMHC-IIA or NMHC-IIB.
