Skip to main content
. Author manuscript; available in PMC: 2010 Dec 7.
Published in final edited form as: Cell Stem Cell. 2009 Dec 4;5(6):624–633. doi: 10.1016/j.stem.2009.10.003

Figure 5. Treatment with GABAA Receptor Antagonist PTX Ameliorates Developmental Alterations of Adult-Born GCs in 2–3-Month-Old hAPP-J20 Mice.

Figure 5

(A, C) Photomicrographs of spines (A) and dendrites (C) of 18-day-old adult-born GCs in hAPP-J20 and NTG mice treated with vehicle or PTX (1.0 mg/kg body weight) from 1–7 dpi. Scale bars: 3 µm (A), 20 µm (C).

(B, D) Quantification of spine densities (B) and dendritic lengths (D) in 18- and 28-day-old adult-born GCs of hAPP and NTG mice treated with vehicle or PTX from 1–7 dpi. A two-way ANOVA analysis revealed significant genotype effects on spine density (B) at 18 dpi (F(1,96) = 70.21, P < 0.0001) and 28 dpi (F(1,184) = 7.067, P = 0.0085), on dendritic length (D) at 18 dpi (F(1,184) = 42.07, P < 0.0001) and 28 dpi (F(1,134) = 9.360, P = 0.0027), significant PTX effects on spine density (B) at 18 dpi (B: F(1,96) = 5.673, P = 0.0192), and significant genotype × PTX interactions for both spine density (B) (F(1,184) = 49.52, P < 27 0.0001) and dendritic length (D) (F(1,134) = 22.05, P < 0.0001) at 28 dpi. *, P < 0.05; **, P < 0.01; ***, P < 0.001, Bonferroni posthoc test.

(E, F) Quantification of spine densities (E) and dendritic lengths (F) in 28-day-old adult-born GCs of hAPP-J20 and NTG mice treated with vehicle or PTX from 14–27 dpi. A two-way ANOVA identified a significant main effect of genotype for both spine density (E: F(1,151) = 52.79, P < 0.0001) and dendritic length (F: F(1,138) = 32.72, P < 0.0001), a significant PTX effects on spine density (E: F(1,151) = 6.038, P = 0.0151), and a significant genotype × PTX interaction for both spine density (E: F(1,151) = 4.550, P = 0.0345) and dendritic length (F: F(1,138) = 4.173, P = 0.0430). *, P < 0.05; ***, P < 0.001, Bonferroni posthoc test. Values are means ± SEM (B, D–F).