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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Mol Cell Neurosci. 2010 Jan 4;43(3):315–325. doi: 10.1016/j.mcn.2009.12.005

Figure 7.

Figure 7

Kv4.2 co-immunoprecipitates with the PKA anchoring protein AKAP79. (A, B) COS7 cells expressing Kv4.2 alone (A, line 4) or coexpressing AKAP79 with either Kv4.2 (A, line1-3) or KChIP4a (B). In co-immunoprecipitation experiments, cell lysates were pulled down using anti-AKAP79 or nonspecific IgG, and probed with anti-Kv4.2 antibody (1:2000) or anti-AKAP79 (1:1000) and anti-Flag antibody (1:6000) to detect KChIP4a, then visualized by anti-mouse Alexa Fluor 680 and anti-rabbit Alexa Fluor 800 secondary antibody, respectively. These results demonstrate that Kv4.2 but not KChIP4a interacts with AKAP79. (C) Images of Kv4.2 and AKAP150 (mouse homologue of AKAP79) in hippocampal showing their co-localization (red, AKAP150; green, Kv4.2; yellow, overlapping signals). Scale bar, 10 μm.