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(A) Schematic of reovirus reverse genetics system using BHK-T7 cells. (B) BHK-T7 cells were co-transfected with four rescue plasmids corresponding to the T1L and T3D genomes. Following 48 h incubation, transfected cells were lysed by freeze-thaw, and viral titers in cell lysates were determined by plaque assay.
