Table 1.
Oligodeoxynucleotide primers used for plasmid construction
| Plasmid DNA | Nucleotide sequencea | |
|---|---|---|
| pT7-L1T1L | Forward | 5'-TAACCCGGGTAATACGACTCACTATAGCTACACGTTCCACGACAATGTCATCCA-3' |
| Reverse | 5'-GGTCGGACCGCGAGGAGGTGGAGATGCCATGCCGACCCGATGAGTTGACGCACCACGACCCAT-3' | |
| pT7-L2T1L | Forward | 5'-TAACCCGGGTAATACGACTCACTATAGCTATTGGCGCAATGGCGAACGTTTGGG-3' |
| Reverse | 5'-GGTCGGACCGCGAGGAGGTGGAGATGCCATGCCGACCCGATGAATTAGGCACGCTCACGAGGG-3' | |
| pT7-L3T1L | Forward | 5'-TAAGAATTCTAATACGACTCACTATAGCTAATCGTCAGGATGAAGCGGATTCCA-3' |
| Reverse | 5'-GGTCGGACCGCGAGGAGGTGGAGATGCCATGCCGACCCGATGAATCGGCCCAACTAGCATCGA-3' | |
| pT7-M2T1L | Forward | 5'-CCTCGCGGTCCGACCTGGGGTTAATACGACTCACTATAGCTAATCTGCTGACCGTCACTCT-3' |
| Reverse | 5'-GGTCGGACCGCGAGGAGGTGGAGATGCCATGCCGACCCGATGATGTGCCTGCACCCTTAACC-3' | |
| pT7-M3T1L | Forward | 5'-TAAGATATCTAATACGACTCACTATAGCTAAAGTGACCGTGGTCATGGCTTCAT-3' |
| Reverse | 5'-GGTCGGACCGCGAGGAGGTGGAGATGCCATGCCGACCCGATGAATAGGGGTCGGGAAGGCTTA-3' | |
| pT7-S2T1L | Forward | 5'-TAACCCGGGTAATACGACTCACTATAGCTATTCGCTGGTCAGTTATGGCT-3' |
| Reverse | 5'-ACCGCGAGGAGGTGGAGATGCCATGCCGACCCGATGAATGTGTGGTCAGTCGTGAGGGGTGTG-3' | |
| pT7-S3T1L | Forward | 5'-TAACCCGGGTAATACGACTCACTATAGCTAAAGTCACGCCTGTTGTCGTCAC -3' |
| Reverse | 5'-GGTCGGACCGCGAGGAGGTGGAGATGCCATGCCGACCCGATGATTAGGCGCCACCCACCACC-3' | |
| pT7-S4T1L | Forward | 5'-TAAGATATCTAATACGACTCACTATAGCTATTTTTGCCTCTTCCCAAACG-3' |
| Reverse | 5'-CGCGAGGAGGTGGAGATGCCATGCCGACCCGATGAATGGAGCCTGTCCCACGTC-3' |
a
The T7 RNA polymerase promoter sequence is underlined.