Figure 3.
Representative immunoblot of Caspase-9 (a) and -3 (c) protein levels in frontal cortex of controls (n = 10) and BD patients (n = 10). Data are ratios of optical density of Caspases to β-actin, expressed as percent of control. mRNA levels of Caspase-9 (b) and -3 (d) in postmortem control (n = 10) and BD (n = 10) frontal cortex, measured using real time RT-PCR. Mean ± SEM, *p < 0.05, ***p < 0.001. (e) Representative histology of control and BD frontal cortex. Active Caspase-3 was detected by kit as described in materials and methods. Scale bar, 100 μm (lower) and 20 μm (upper). Red stains indicated by arrows represent the expression of active Caspase-3 in BD frontal cortex. Counterstaining was performed using methyl green.