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Details of stepping. Histograms show motor domain separation in raw images (gray bars) and image averages (black bars). Raw image distances were measured between motors using SPIDER. Distances between heads in image averages were measured between the attached actins. (Insets) Class averages obtained by segregating images according to motor separation. Classification used reference-free methods after windowing and aligning myosins attached by both heads. (Scale bar, 50 nm.)
