Fig. 5.

Effects of gene disruption on experimental metastasis. Cells with the indicated genotypes were injected into the spleens of immunodeficient mice, who were killed 5 weeks later. Five mice were injected with each cell line. (A) Number of metastatic foci visible on the liver surface on necropsy. There were at least 200 foci in parental HC116 cells, but the actual number was difficult to count because of adjoining lesions (Fig. S3). (B) Amount of human tumor DNA in the livers. (C) Amount of human tumor DNA in the spleens.