Fig. 1.

Paxillin is a direct substrate of PTPRT. (A) Paxillin is pulled down by substrate-trapping mutants of PTPRT. Colon cancer cell lysates were incubated with beads bound to the indicated GST-fusion proteins. Western blots were performed with an anti-paxillin antibody. (B) PTPRT dephosphorylates paxillin at residue Y88. Phospho-paxillin proteins were immunoprecipitated from lysate of HEK 293 cells. The immunocomplexes were incubated with the indicated recombinant proteins with or without Na₃VO₄. (C) DLD1 cells were infected with adenoviruses expressing PTPRT or GFP and starved and then stimulated with PDGF-AA for the indicated times. The intensity of pY88 signals were quantified with NIH image and normalized against total paxillin levels. Fold change of pY88 paxillin for each time point over unstimulated PTPRT-infected cells was calculated.