Fig. 2.

Paxillin Y88F mutant CRC cells are less tumorigenic. (A) Anchorage-independent growth. CRC cells of the indicated clones were mixed in 0.4% soft agar and plated in six-well plates in triplicates. Cells were grown for 30 days, and colony foci were counted (*, P < 0.001; **, P < 0.05). (B) Athymic nude mice were injected s.c. and bilaterally with cells of the indicated genotypes and killed 35 days after cell injection. Mice that formed xenografts of the indicated genotypes were counted. (C) Tumor sizes of the indicated clones were measured weekly for 5 weeks, and the average volume at each time point was plotted.