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Paxillin Y88F mutant affect cell signaling through altering phosphorylation of AKT, p130CAS, and SHP2. Parental (WT) and paxillin Y88F homozygous KI CRC cells were serum-starved for 16 h and stimulated with PDGF-AA for the indicated times. Western blot analyses were performed with the indicated antibodies.
