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. 2010 Jan 21;107(6):2491–2496. doi: 10.1073/pnas.0909080107

Fig. 1.

Fig. 1.

SYTA binds Begomovirus MP and TMV 30K in vitro. (A) Annotated SYTA locus (Upper) and predicted SYTA domain structure (Lower). Shown are 5′- and 3′-UTRs, exons (boxes), nucleotides in genomic locus (Upper) and in full-length cDNA (Lower), and syta-1 (Sail775A08) T-DNA insertion (cDNA nt 1,728). TM, transmembrane domain. (B) SDS/PAGE analysis of 35S-met-labeled MPCaLCuV or MPSqLCV (33 kDa), TMV 30K [32–34 kDa doublet (5)], or luciferase (61 kDa) bound (BND) to GST-SYTAΔTM or GST in vitro, and 8% of unbound proteins (UN). Small MPCaLCuV products are from two internal AUG starts.