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. 2010 Jan 21;107(6):2521–2526. doi: 10.1073/pnas.0914396107

Fig. 2.

Fig. 2.

Organ laterality is randomized in setdb2-knockdown embryos. (A–L) Whole-mount mRNA in situ hybridization and statistical analyses of visceral and diencephalic laterality in the 5-MM control and setdb2 MO2-injected embryos at the indicated developmental stages with probes cmlc2 (A–C), lefty1 (DF), cebpα (GI, arrows), and insulin (JL). (MO) Determination of pancreatic laterality in 72-hpf Tg(insulin:EGFP) transgenic embryos injected with 5-MM control and setdb2 MO2. Dashed lines denote the embryonic midline. LV, liver; G, gut; L, Left side; R, Right side; C/B, central or bilateral; A, absent. All embryos are dorsal views with head to the top.