Organ laterality is randomized in setdb2-knockdown embryos. (A–L) Whole-mount mRNA in situ hybridization and statistical analyses of visceral and diencephalic laterality in the 5-MM control and setdb2 MO2-injected embryos at the indicated developmental stages with probes cmlc2 (A–C), lefty1 (D–F), cebpα (G–I, arrows), and insulin (J–L). (M–O) Determination of pancreatic laterality in 72-hpf Tg(insulin:EGFP) transgenic embryos injected with 5-MM control and setdb2 MO2. Dashed lines denote the embryonic midline. LV, liver; G, gut; L, Left side; R, Right side; C/B, central or bilateral; A, absent. All embryos are dorsal views with head to the top.