Fig. 5.

Inducible BDNF deletion in PL with Cre lentivirus. (A and B) Robust lentiviral Cre expression was labeled by LacZ when injected into Rosa LacZ reporter mice in striatum. (C) Cre recombinase mRNA expression showed the infection site targeting the PL. (D) BDNF mRNA was specifically deleted in the PL and (E) overlays with the infection of Cre recombinase lentivirus. Red, BDNF mRNA; green, Cre mRNA. LV-GFP control infections in the PL of fBDNF mice at (F) 40× magnification and (G) 100× magnification. Nissl stain with cresyl violet showed no appreciable cell damage after lentiviral infection of (H) LV-GFP or (I) LV-Cre. (Scale bars: 500 μm.)