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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Dev Biol. 2009 Dec 21;339(1):188–199. doi: 10.1016/j.ydbio.2009.12.012

FIGURE 2. CNS-mediated inputs and GJC are required early during phases of regeneration.

FIGURE 2

(A) The percentage of bipolar-head regeneration was recorded from post-pharyngeal fragments in which octanol treatment began at different time points post-amputation. The incidence of bipolar-head phenotype was sharply reduced when GJ blocker (octanol) treatment began a few hours post-amputation, suggesting that critical decisions to identify missing parts and set A/P polarity take place early (within first 3-6 hours) during regeneration. (B) Planarians were amputated at post-pharyngeal level, treated with octanol, and subjected to disruption of VNC at anterior areas (red dotted line in pre-pharyngeal area) at different time points after initial post-pharyngeal cut (time 0). Significant A/P duplications were observed when VNC contiguity was disrupted prior to 12 hours after amputation. Numbers in parenthesis indicate the sequence of amputations, being first post-pharyngeal and then VNC disruption at pre-pharyngeal level; all animals were treated with octanol. For each time point n≥ 10 animals. In all cases data are represented as mean +/− confidence intervals (95%) and curve fitting is shown with dotted line.