Table 2.

k_cat/K_m values for potential guanidinium containing substrates of Wild Type and Mutant HpAgD

Substrate	Wild Type (min−1 M−1)	C324A (min−1 M−1)	D198A (min−1 M−1)
H4d,f	≤ 8.55×10−6	NDa	≤ 2.58×10−7
L-Arginineb,f	≤ 1.03	NDa	≤ 8.96
Streptomycinb,e	≤ 2.26	NDa	≤ 1.98
Ac-H4-5c,e	≤ 0.23	NDa	≤ 1.41
BAAb,e	≤ 4.32	NDa	≤ 7.71
Guanidineb,e	≤ 4.87	NDa	≤ 4.07
BAEEb,f	≤ 5.08	NDa	≤ 8.30
BAMEb,f	≤ 6.23	NDa	≤ 13.1
ADMAb,f	≤ 17.2	NDa	≤ 1.46
Agmatine	147 ×103	≤ 19.2	≤ 1.7×103

^aND=Not Determined;

^b10 mM Substrate;

^c5 mM Substrate;

^d125 μM Substrate;

^e2 h incubation;

^f3 h incubation.