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Steady-State Kinetic Parameters for Agmatine with Wild Type and Mutant HpAgD
| Enzyme | Substrate | kcat (min−1) | Km (μM) | kcat/Km (min−1M−1) |
|---|---|---|---|---|
| Wild Type | Agmatine | 4.90 ± 0.29 | 33 ± 8.8 | 147 ×103 |
| C324Aa | Agmatinea | NDb | NDb | ≤ 19.2 |
| D198Aa | Agmatine | 0.28 ± 0.01 | 170 ± 79.8 | 1.7×103 |
10 mM agmatine,
ND=Not Determined.
