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. Author manuscript; available in PMC: 2011 Mar 17.
Published in final edited form as: Neuroscience. 2009 Dec 28;166(2):680–697. doi: 10.1016/j.neuroscience.2009.12.053

Figure 9.

Figure 9

High magnification photomicrographs of NKB (A and E, green) and BDA (B and F; red) immunofluorescence. The color-combined images (C and G) show that these NKB-immunoreactive axons are anterogradely labeled with BDA (yellow). The map in D shows the location of these dual-labeled axons. The top photomicrographs illustrate a thin, beaded anterogradely-labeled NKB-ir axon extending to the lateral ventral septal region (A, B and C, arrowheads). The bottom photomicrographs illustrate a dual-labeled axon in the medial preoptic region that is thicker and displays few varicosities (E, F, and G, arrowheads). The arrows show single-labeled fibers that are immunoreactive for either BDA (B and C) or NKB (E and G). Scale bar in A = 10 μm (applies to A-C and E-F). Scale bar in D = 200 μm.