A. Fifty micrograms of cell extracts were separated on 10% SDS-PAGE and subjected to immunoblotting using antibodies specific to Rab1A, 2, 4A, 5A, 5B, 6 and 7 proteins. B. Quantitation of the band volume of each Rab protein in the C5B bound fraction relative to C5B lysate. Notice the preferential enrichment of Rab2, 6 and 7 in the C5B bound fraction. Results are the average from two independent experiments.