Male C57Bl/6 mice were exposed to Western diet (WD) and control diet (C-WD) for 21 weeks, and the effects of diet on brain levels of the cytokine TNFα and IL-6, the chemokine MCP-1, and the growth factor BDNF were evaluated by ELISA as described in Methods. Data are means and SEM, with 8–10 individual mice per group, and statistical analyses (two-tailed, unpaired t-tests) indicated no significant effects of diet on cytokine, chemokine, or growth factor expression in the cortex.