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. Author manuscript; available in PMC: 2011 Feb 26.
Published in final edited form as: J Neuroimmunol. 2009 Dec 8;219(1-2):25–32. doi: 10.1016/j.jneuroim.2009.11.010

Figure 3. Effects of WD on glial marker expression in mouse brain.

Figure 3

Male C57Bl/6 mice were exposed to Western diet (WD) and control diet (C-WD) for 21 weeks, and the effects of diet on expression of the astrocyte marker GFAP and the microglia marker Iba-1 were measured by Western blot. Individual samples were compared to tubulin as loading controls, and 8–10 mice per group were evaluated. Representative blot images depict the ability of WD to increase GFAP, but not Iba-1, expression in mouse brain. Data are means and SEM, with 8–10 individual mice per group, and statistical analyses (two-tailed, unpaired t-tests) indicated a significant effect of WD on GFAP (* p < 0.05) levels in the cortex.